 
															Wildlife Conservation Laboratory- Los Amigos
				Wildlife Conservation Laboratory- Los Amigos
Located at the Estación Biológica Río Los Amigos, Madre de Dios, Peru. Operated by Conservación Amazónica – ACCA.
MADRE DE DIOS, PERU
KIGALI, RWANDA
Each with their own particular style, these laboratories are different examples of a One Health ISL approach
 
															Located at the Estación Biológica Río Los Amigos, Madre de Dios, Peru. Operated by Conservación Amazónica – ACCA.
Located at Umusambi Village, Kigali, Rwanda. Operated by the Rwanda Wildlife Conservation Association.
 
															Madre de Dios, Peru
| TIMELINE | Design and planning commenced September 2020 → Renovation of existing building, April to June 2021→ Installation of 20 kW solar system in June 2021 → Equipment installed in July 2021 | 
 
															 
															 
															Figure 1: WCL-Los Amigos showing A) the interior before renovation, and B) and C) the same room divided into isolated laboratory work spaces.
 
															Figure 2: The floor plan at the WCL – Los Amigos. Please see layouts for full details.
| ITEM | DESCRIPTION | 
|---|---|
| Initiated in: | 2021 | 
| Location | Lowland Amazonian Rainforest | 
| Access | River | 
| AREA | ~120 meters-sq | 
| Energy | Solar: avg. of 56 (+/- 15 kWh) per day, calc. over 3-months | 
| Max AC power | 110v (up to 5 kW) ; 220v (up to 10 kW) | 
| Back-up power | 20 kW diesel-fuel generator | 
| Batteries | 24 200A 12V batteries (non-spillable / gel type) | 
| Divisions | 5 designated working spaces: (1) sample intake and storage room (2) nucleic acid extraction room (3) DNA free room AKA the “clean room” (4) general laboratory space (5) toxicology and conservation technology room | 
| Automation | Yes | 
| Sequencing Platform | Oxford Nanopore Mk1c & P2-Solo | 
| Internet service | Starlink | 
| Applications | -Wildlife parasite and pathogen screening -Species molecular classification -Species population genetics -Mitochondrial genome sequencing -Nuclear genome sequencing -Metabarcoding (diet and microbiome) | 
| Budget | TBA | 
Kigali, Rwanda
| TIMELINE | Design and planning commenced October 2022 → Installation and renovation of shipping containers into laboratory space, December 2022 to March 2023→ Energy connection and equipment installation completed in June 2023 | 
 
															 
															Figure 1: A) A view of compartmentalized laboratory spaces to minimise contamination and B) Team members outside the new laboratory.
 
															
Figure 2: The floor plan at the Rwanda WCL. Please see layouts for full details.
| ITEM | DESCRIPTION | 
|---|---|
| Initiated in: | 2023 | 
| Location | Marshland habitat | 
| Access | Road | 
| Area | 30 meters-sq (1.5 standard shipping containers) | 
| Energy | Grid | 
| Max AC power | 110v (up to 5kW) ; 220v | 
| Back-up power | NA | 
| Batteries | NA | 
| Divisions | 4 designated working spaces: (1) sample intake and nucleic acid extraction room (2) PCR room and electrophoresis room (3) DNA free and sequencing room (4) Sterilization room | 
| Automation | No | 
| Sequencing Platform | Oxford Nanopore Mk1b | 
| Internet service | Local provider | 
| Applications | Parasite and pathogen screening Species molecular classification | 
| Budget | TBA |